Abstract
We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples (out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriological Techniques / methods*
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Base Sequence
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DNA Primers / genetics
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Escherichia coli / genetics
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Escherichia coli / isolation & purification*
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Escherichia coli Infections / diagnosis*
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Humans
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Molecular Sequence Data
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Nucleic Acid Amplification Techniques / methods*
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Sensitivity and Specificity
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Urine / microbiology