Development of a serotyping ELISA system for Thailand virus infection

Arch Virol. 2008;153(8):1537-42. doi: 10.1007/s00705-008-0128-4. Epub 2008 Jun 13.

Abstract

To distinguish Thailand virus infection from infections with other hantaviruses, we established an ELISA serotyping system using a truncated nucleocapsid protein of Thailand virus lacking 49 amino acids at the N-terminus. In evaluations using patient and rodent sera, Thailand virus infection was readily distinguished from Hantaan and Seoul virus infections. Therefore, this ELISA system is an effective alternative to neutralization tests.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral / immunology
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Hantavirus Infections / diagnosis*
  • Hantavirus Infections / immunology
  • Humans
  • Immune Sera / immunology
  • Neutralization Tests
  • Nucleocapsid Proteins / immunology
  • Orthohantavirus / classification*
  • Orthohantavirus / isolation & purification
  • Serotyping*
  • Thailand

Substances

  • Antibodies, Viral
  • Immune Sera
  • Nucleocapsid Proteins