Abstract
Using culture-independent technology, PCR-RFLP were used to identify and type STEC in the stool of a patient with HUS. Fecal PCR-RFLP patterns were identical to those of the STEC O157:H7 isolated from the patient.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Typing Techniques
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DNA, Bacterial / genetics
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Diagnosis, Differential
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Diarrhea / diagnosis*
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Diarrhea / microbiology
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Escherichia coli Infections / diagnosis*
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Escherichia coli Infections / microbiology
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Escherichia coli O157 / genetics
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Escherichia coli O157 / isolation & purification*
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Feasibility Studies
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Feces / microbiology*
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Hemolytic-Uremic Syndrome / diagnosis*
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Hemolytic-Uremic Syndrome / microbiology
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Humans
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Polymerase Chain Reaction*
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Polymorphism, Restriction Fragment Length*
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Shiga-Toxigenic Escherichia coli / classification*
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Shiga-Toxigenic Escherichia coli / genetics
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Shiga-Toxigenic Escherichia coli / isolation & purification*