Rapid culture-free identification and molecular typing of Shiga toxin-producing Escherichia coli by PCR-RFLP

Microbiol Immunol. 2008 Jun;52(6):310-3. doi: 10.1111/j.1348-0421.2008.00038.x.

Abstract

Using culture-independent technology, PCR-RFLP were used to identify and type STEC in the stool of a patient with HUS. Fecal PCR-RFLP patterns were identical to those of the STEC O157:H7 isolated from the patient.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • DNA, Bacterial / genetics
  • Diagnosis, Differential
  • Diarrhea / diagnosis*
  • Diarrhea / microbiology
  • Escherichia coli Infections / diagnosis*
  • Escherichia coli Infections / microbiology
  • Escherichia coli O157 / genetics
  • Escherichia coli O157 / isolation & purification*
  • Feasibility Studies
  • Feces / microbiology*
  • Hemolytic-Uremic Syndrome / diagnosis*
  • Hemolytic-Uremic Syndrome / microbiology
  • Humans
  • Polymerase Chain Reaction*
  • Polymorphism, Restriction Fragment Length*
  • Shiga-Toxigenic Escherichia coli / classification*
  • Shiga-Toxigenic Escherichia coli / genetics
  • Shiga-Toxigenic Escherichia coli / isolation & purification*

Substances

  • DNA, Bacterial