Surface AFM microscopy of unworn and worn samples of silicone hydrogel contact lenses

J Biomed Mater Res B Appl Biomater. 2009 Jan;88(1):75-82. doi: 10.1002/jbm.b.31153.

Abstract

Purpose: To evaluate the qualitative and quantitative topographic changes in the surface of worn contact lenses (CLs) of different materials using atomic force microscopy (AFM).

Methods: The topography of five different CL materials was evaluated with AFM over a surface of 25 microm(2) according to previously published experimental setup. Average roughness (R(a)) and root mean square (Rms) values were obtained for unworn and worn samples.

Results: The R(a) value increased for balafilcon A (11.62-13.68 nm for unworn and worn samples, respectively), lotrafilcon A (3.67-15.01 nm for unworn and worn samples, respectively), lotrafilcon B (4.08-8.42 nm for unworn and worn samples, respectively), galyfilcon A (2.81-14.6 nm for unworn and worn samples, respectively), and comfilcon A (2.87-4.63 nm for unworn and worn samples, respectively). Differences were statistically significant for all lenses except Rms and R(a) for comfilcon A, and R(a) parameter for balafilcon A (p > 0.05). The least relative increase was observed for some balafilcon A samples and for some of these samples the roughness decreased after the lenses had been worn.

Conclusion: The changes in surface roughness between unworn and worn lenses are different for different silicone-hydrogel materials. Overall all CLs increased the degree of surface roughness after being worn, even for very short periods of time. However, for samples of balafilcon A, roughness increases at a lower extent or even can decrease as compared to unworn samples of the same material due to filling of the macropores.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Contact Lenses*
  • Contact Lenses, Hydrophilic*
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Hydrogels / chemistry*
  • Materials Testing
  • Microscopy, Atomic Force / methods*
  • Pseudomonas aeruginosa / metabolism
  • Silicon / chemistry*
  • Silicones / chemistry
  • Surface Properties
  • Wettability

Substances

  • Hydrogels
  • Silicones
  • balafilcon A
  • comfilcon A
  • galyfilcon A
  • lotrafilcon A
  • lotrafilcon B
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Silicon