Disposable microscope objective lenses for fluorescence correlation spectroscopy using latex microspheres

Anal Chem. 2008 Sep 1;80(17):6800-4. doi: 10.1021/ac801016z. Epub 2008 Aug 6.

Abstract

We explore the combination of a latex microsphere with a low NA lens to form a high performance optical system, and enable the detection of single molecules by fluorescence correlation spectroscopy (FCS). Viable FCS experiments at concentrations 1-1000 nM with different objectives costing less than $40 are demonstrated. This offers a simple and low-cost alternative to the conventional complex microscope objectives.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Costs and Cost Analysis
  • Disposable Equipment* / economics
  • Latex*
  • Lenses* / economics
  • Microscopy / economics
  • Microscopy / instrumentation*
  • Microspheres*
  • Spectrometry, Fluorescence / instrumentation*

Substances

  • Latex