Phospholipase C isozymes in neurons and glial cells in culture: an immunocytochemical and immunochemical study

Brain Res. 1991 May 10;548(1-2):35-40. doi: 10.1016/0006-8993(91)91103-8.

Abstract

Expression of 3 brain isozymes of phospholipase (PLC-beta, PLC-gamma and PLC-delta) was investigated in relation to cell types found in rat CNS cultures. Immunoreactivity of cultured neurons, astrocytes and oligodendrocytes was demonstrated for all of the 3 isozymes by immunocytochemical staining and immunoblotting, with some differences in reaction intensity. Immunoblotting revealed that the level of expression was neurons greater than oligodendrocytes greater than astrocytes for PLC-beta and PLC-gamma, and astrocytes greater than oligodendrocytes greater than neurons for PLC-delta.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / enzymology*
  • Cells, Cultured
  • Fetus
  • Fluorescent Antibody Technique
  • Immunoblotting
  • Immunohistochemistry
  • Isoenzymes / analysis*
  • Neuroglia / cytology
  • Neuroglia / enzymology*
  • Neurons / cytology
  • Neurons / enzymology*
  • Rats
  • Rats, Inbred Strains
  • Spinal Cord / enzymology*
  • Type C Phospholipases / analysis*

Substances

  • Isoenzymes
  • Type C Phospholipases