Nuruk extract inhibits lipopolysaccharide-induced production of nitrite and interleukin-6 in RAW 264.7 cells through blocking activation of p38 mitogen-activated protein kinase

J Microbiol Biotechnol. 2008 Aug;18(8):1423-6.

Abstract

Nuruk, which is a natural inoculator and source of amylolytic enzymes, is used in Korean traditional rice wine. A methanol extract of nuruk (NE) attenuated lipopolysaccharide (LPS)- induced nitrite and interleukin (IL)-6 in RAW 264.7 cells. Both the n-hexane and water fractions from NE (MEH and MW, respectively) inhibited the production of nitrite and IL-6 in RAW 264.7 cells. MEH and MW also inhibited the LPS-induced inducible nitric oxide synthase expression. Further, and MEH protected against the LPS-induced activation of p38 mitogen-activated protein kinase. Together, these results indicate that nuruk may contribute to the anti-inflammatory and cancer-preventive effects of Korean traditional rice wine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Enzyme Activation / drug effects
  • Fungi / chemistry*
  • Fungi / metabolism
  • Interleukin-6 / biosynthesis*
  • Lipopolysaccharides / antagonists & inhibitors*
  • Lipopolysaccharides / metabolism
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects*
  • Macrophages / enzymology
  • Macrophages / metabolism
  • Mice
  • Nitric Oxide Synthase Type II / metabolism
  • Nitrites / metabolism*
  • p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors
  • p38 Mitogen-Activated Protein Kinases / metabolism*

Substances

  • Interleukin-6
  • Lipopolysaccharides
  • Nitrites
  • Nitric Oxide Synthase Type II
  • p38 Mitogen-Activated Protein Kinases