Interleukin-10 is an essential modulator of mucoid metaplasia in a mouse otitis media model

Ann Otol Rhinol Laryngol. 2008 Aug;117(8):630-6. doi: 10.1177/000348940811700814.

Abstract

Objectives: Inflammatory cytokines are involved in the development of mucous cell metaplasia and hyperplasia (MCM) in otitis media (OM). However, which cytokines play an essential role in the MCM of OM is not clear at the moment.

Methods: In this study, we hypothesized that interleukin-10 (IL-10) played an indispensable role in the MCM of bacterial OM, and we used IL-10 knockout mice to test this hypothesis.

Results: In wild-type mice, both Streptococcus pneumoniae and Haemophilus influenzae triggered the development of MCM in the middle ear mucosa. In IL-10 knockout mice, the number of goblet cells and mucin-producing cells in the middle ear was significantly reduced after bacterial middle ear infection compared with that in wild-type mice.

Conclusions: We conclude that IL-10 plays an essential role in the MCM of bacterial OM. Interleukin-10 is a potential target for the treatment of MCM in OM.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Disease Models, Animal*
  • Ear Neoplasms / drug therapy*
  • Ear Neoplasms / metabolism
  • Ear Neoplasms / pathology*
  • Ear, Middle / drug effects*
  • Ear, Middle / pathology
  • Goblet Cells / drug effects
  • Goblet Cells / metabolism
  • Goblet Cells / pathology
  • Haemophilus Infections / complications
  • Haemophilus influenzae
  • Interleukin-10 / therapeutic use*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mucins / metabolism
  • Mucous Membrane / drug effects*
  • Mucous Membrane / metabolism
  • Mucous Membrane / pathology*
  • Otitis Media / drug therapy*
  • Otitis Media / microbiology
  • Otitis Media / pathology*
  • Rats
  • Rats, Sprague-Dawley
  • Streptococcal Infections / complications
  • Streptococcus pneumoniae

Substances

  • Mucins
  • Interleukin-10