Effects of sodium butyrate on the rearranged c-myc expression in mouse plasmacytoma cells

Exp Cell Res. 1991 Sep;196(1):146-9. doi: 10.1016/0014-4827(91)90469-b.

Abstract

The expression of c-myc mRNA was examined after 4 h of sodium butyrate treatment in a mouse plasmacytoma (MPC) cell line (S194). Steady-state levels of rearranged c-myc mRNA were suppressed by the agent in S194 cells. Run-on assay demonstrated that the suppression of the rearranged c-myc mRNA in the MPC was correlated with the transcriptional downregulation of the gene. The suppression was also accompanied by the reduced DNase I sensitivity of the gene. These findings suggest that the rapid downregulation of c-myc mRNA by sodium butyrate is subject to regulation at the transcriptional level following the alteration of the DNase I sensitive chromatin structure in mouse plasmacytoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Butyrates / pharmacology*
  • Butyric Acid
  • Chromatin / drug effects
  • Chromatin / ultrastructure
  • DNA, Neoplasm / drug effects
  • Deoxyribonuclease I / pharmacology
  • Down-Regulation / drug effects
  • Gene Expression Regulation, Neoplastic / drug effects
  • Gene Rearrangement / physiology
  • Genes, myc / drug effects
  • Genes, myc / genetics
  • Mice
  • Mice, Inbred BALB C
  • Plasmacytoma / metabolism
  • Plasmacytoma / pathology*
  • Proto-Oncogene Proteins c-myc / genetics
  • Proto-Oncogene Proteins c-myc / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Transcription, Genetic / drug effects
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / metabolism
  • Tumor Cells, Cultured / pathology

Substances

  • Butyrates
  • Chromatin
  • DNA, Neoplasm
  • Proto-Oncogene Proteins c-myc
  • RNA, Messenger
  • Butyric Acid
  • Deoxyribonuclease I