RNA intramolecular dynamics by single-molecule FRET

Curr Protoc Nucleic Acid Chem. 2008 Sep:Chapter 11:Unit 11.12. doi: 10.1002/0471142700.nc1112s34.

Abstract

Investigation of single RNA molecules using fluorescence resonance energy transfer (FRET) is a powerful approach to investigate dynamic and thermodynamic aspects of the folding process of a given RNA. Its application requires interdisciplinary work from the fields of chemistry, biochemistry, and physics. The present work gives detailed instructions on the synthesis of RNA molecules labeled with two fluorescent dyes interacting by FRET, as well as on their investigation by single-molecule fluorescence spectroscopy.

MeSH terms

  • Fluorescence Resonance Energy Transfer / instrumentation
  • Fluorescence Resonance Energy Transfer / methods*
  • Fluorescent Dyes / chemistry
  • Lasers
  • Nucleic Acid Conformation
  • RNA / chemical synthesis
  • RNA / chemistry*
  • Spectrometry, Fluorescence
  • Thermodynamics

Substances

  • Fluorescent Dyes
  • RNA