[Establishment and characterization of a CA19-9 producing human gastric cancer cell line, STKM-1]

Hum Cell. 1991 Mar;4(1):67-70.
[Article in Japanese]

Abstract

A human gastric cancer cell line, STKM-1, was established from the malignant cells in pleural effusion of a 41-year-old female patient. The primary gastric cancer revealed histologically a poorly differentiated adenocarcinoma. The cells have been cultured with RPMI-1640 medium supplemented with 10% fetal bovine serum and grew as monolayers following a doubling time of 31.4 hour at passage 30. The mode of chromosome number was 52. The STKM-1 cell was tumorigenic in nude mice. The STKM-1 cell cultured in vitro secreted CA19-9, into the medium as a tumor marker. Cells in tumors grown in nude mice were immunohistochemically recognized positively by anti-CA19-9 antibody. The STKM-1 will provide a useful information to clarify the mechanism of CA19-9 secretion.

Publication types

  • Case Reports
  • English Abstract

MeSH terms

  • Adenocarcinoma / immunology
  • Adenocarcinoma / pathology*
  • Adult
  • Animals
  • Antigens, Tumor-Associated, Carbohydrate / biosynthesis*
  • Biomarkers, Tumor / biosynthesis*
  • Cell Division
  • Female
  • Humans
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Stomach Neoplasms / immunology
  • Stomach Neoplasms / pathology*
  • Tumor Cells, Cultured*

Substances

  • Antigens, Tumor-Associated, Carbohydrate
  • Biomarkers, Tumor