[Phosphorylation and nuclear translocation of serine 722 and serine 910 of focal adhesion kinase in hypertrophic cardiac myocytes of left ventricle of spontaneously hypertensive rats]

Zhonghua Bing Li Xue Za Zhi. 2008 May;37(5):328-32.
[Article in Chinese]

Abstract

Objective: To investigate the role of focal adhesion kinase (FAK) in cardiac hypertrophy induced by hypertension.

Methods: Using immunofluorescent labeling, confocal microscopy and Western blot, the expression and subcellular location of FAK-pSer722 and FAK-pSer910 were determined in cardiac myocytes of the left ventricles from 2, 6, 12, and 18 month-old spontaneously hypertensive heart failure (SHHF) rats and age-matched Wistar-Kyoto (WKY) control rats, respectively.

Results: There was no obvious difference in FAK-pSer722 and FAK-pSer910 expression between 2 month-old SHHF and WKY rats. In contrast with the control groups, the expression of FAK-pSer722 and FAK-pSer910 significantly increased in cardiac myocytes of the left ventricle, from 6, 12 and 18 month-old SHHF rats. Both FAK-pSer722 and FAK-pSer910 were translocated and acummulated in nuclei of cardiac myocytes from 6, 12, and 18 month-old SHHF rats.

Conclusion: Phosphorylation and translocation of serine 722 and serine 910 of phosphorylated FAK play an important role in the de-compensatory cardiac hypertrophy.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Cardiomegaly / enzymology*
  • Cardiomegaly / metabolism
  • Cell Nucleus / enzymology*
  • Cell Nucleus / metabolism
  • Focal Adhesion Kinase 1 / metabolism*
  • Focal Adhesion Protein-Tyrosine Kinases / metabolism
  • Focal Adhesion Protein-Tyrosine Kinases / physiology
  • Heart Failure
  • Heart Ventricles / pathology*
  • Hypertension / enzymology*
  • Hypertrophy / enzymology
  • Myocytes, Cardiac / enzymology*
  • Myocytes, Cardiac / pathology
  • Phosphorylation
  • Protein Transport / drug effects
  • Protein Transport / physiology
  • Rats
  • Rats, Inbred SHR
  • Rats, Inbred WKY
  • Serine / metabolism*
  • Signal Transduction / drug effects*
  • Signal Transduction / physiology

Substances

  • Serine
  • Focal Adhesion Kinase 1
  • Focal Adhesion Protein-Tyrosine Kinases