Salinimicrobium terrae sp. nov., isolated from saline soil, and emended description of the genus Salinimicrobium

Int J Syst Evol Microbiol. 2008 Nov;58(Pt 11):2501-4. doi: 10.1099/ijs.0.65860-0.

Abstract

A novel yellow-pigmented, Gram-negative, slightly halophilic, catalase-positive, oxidase-negative, obligately aerobic bacterium, designated strain YIM-C338(T), was isolated from saline soil from the Qaidam Basin in north-west China. Cells were non-sporulating, non-motile, short rods, predominantly occurring singly. Coccoid bodies and slightly curved rod-shaped cells of varying length developed in older cultures. Growth occurred with 0.5-8 % (w/v) NaCl (optimally with 1-3 %, w/v), at pH 6.0-9.0 (optimally at pH 7.0) and at 4-37 degrees C (optimally at 28 degrees C). The major cellular fatty acids were C(16 : 0), C(18 : 0), C(16 : 1)omega7c, C(18 : 1)omega7c, iso-C(13 : 0) 3-OH and iso-C(15 : 1). MK-6 was the only respiratory quinone. Non-diffusible carotenoid pigments were produced. Flexirubin-type pigments were absent. The genomic DNA G+C content was 42.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM-C338(T) was a member of the genus Salinimicrobium, having sequence similarities of 97.3 and 94.0 % with respect to Salinimicrobium xinjiangense BH206(T) and Salinimicrobium catena HY1(T). The combination of the phylogenetic data, phenotypic characteristics and chemotaxonomic differences supported the view that strain YIM-C338(T) represents a novel species of the genus Salinimicrobium, for which the name Salinimicrobium terrae sp. nov. is proposed. The type strain is YIM-C338(T) (=DSM 17865(T) =CGMCC 1.6308(T)). An emended description of the genus Salinimicrobium is also provided.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • China
  • DNA, Bacterial / analysis
  • DNA, Ribosomal / analysis
  • Flavobacteriaceae / classification*
  • Flavobacteriaceae / genetics
  • Flavobacteriaceae / isolation & purification
  • Flavobacteriaceae / physiology
  • Molecular Sequence Data
  • Phenotype
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sodium Chloride*
  • Soil Microbiology*
  • Species Specificity

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S
  • Sodium Chloride

Associated data

  • GENBANK/EU135614