Male-specific association between a gamma-secretase polymorphism and premature coronary atherosclerosis

PLoS One. 2008;3(11):e3662. doi: 10.1371/journal.pone.0003662. Epub 2008 Nov 6.

Abstract

Background: Atherosclerosis is a common multifactorial disease resulting from an interaction between susceptibility genes and environmental factors. The causative genes that contribute to atherosclerosis are elusive. Based on recent findings with a Wistar rat model, we speculated that the gamma-secretase pathway may be associated with atherosclerosis.

Methodology/principal findings: We have tested for association of premature coronary atherosclerosis with a non-synonymous single-nucleotide polymorphism (SNP) in the gamma-secretase component APH1B (Phe217Leu; rs1047552), a SNP previously linked to Alzheimer's disease. Analysis of a Dutch Caucasian cohort (780 cases; 1414 controls) showed a higher prevalence of the risk allele in the patients (odds ratio (OR) = 1.35), albeit not statistically different from the control population. Intriguingly, after gender stratification, the difference was significant in males (OR = 1.63; p = 0.033), but not in females (OR = 0.50; p = 0.20). Since Phe217Leu-mutated APH1B showed reduced gamma-secretase activity in mouse embryonic fibroblasts, the genetic variation is likely functional.

Conclusion/significance: We conclude that, in a male-specific manner, disturbed gamma-secretase signalling may play a role in the susceptibility for premature coronary atherosclerosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Age of Onset
  • Amino Acid Substitution
  • Animals
  • Cell Line
  • Cohort Studies
  • Coronary Artery Disease / epidemiology
  • Coronary Artery Disease / genetics*
  • Endopeptidases
  • Female
  • Fibrinogen / analysis
  • Fibroblasts / metabolism
  • Humans
  • Male
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Mice
  • Middle Aged
  • Netherlands / epidemiology
  • Peptide Hydrolases
  • Polymorphism, Single Nucleotide*
  • Prevalence
  • Recombinant Fusion Proteins / metabolism
  • Risk Factors
  • Sex Distribution
  • Sex Factors
  • Substrate Specificity
  • White People / genetics

Substances

  • Membrane Proteins
  • Recombinant Fusion Proteins
  • Fibrinogen
  • APH1A protein, human
  • Endopeptidases
  • Peptide Hydrolases