Cloning and expression pattern of a putative octopamine/tyramine receptor in antennae of the noctuid moth Mamestra brassicae

Cell Tissue Res. 2009 Feb;335(2):455-63. doi: 10.1007/s00441-008-0722-5. Epub 2008 Nov 26.

Abstract

In insects, biogenic amines have been shown to play an important role in olfactory plasticity. In a first attempt to decipher the underlying molecular mechanisms, we report the molecular cloning and precise expression pattern of a newly identified octopamine/tyramine-receptor-encoding gene in the antennae of the noctuid moth Mamestra brassicae (MbraOAR/TAR). A full-length cDNA has been obtained through homology cloning in combination with rapid amplification of cDNA ends/polymerase chain reaction; the deduced protein exhibits high identities with previously identified octopamine/tyramine receptors in other moths. In situ hybridization within the antennae has revealed that MbraOAR/TAR is expressed at the bases of both pheromone-sensitive and non-sensitive olfactory sensilla and in cells with a neurone-like shape. In accordance with previous physiological studies that have revealed a role of biogenic amines in the electrical activity of the receptor neurones, our results suggest that biogenic amines (either octopamine or tyramine) target olfactory receptor neurones to modulate olfactory coding as early as the antennal level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Gene Expression / genetics
  • Gene Expression / physiology
  • Molecular Sequence Data
  • Moths / genetics
  • Moths / metabolism*
  • Olfactory Receptor Neurons / cytology
  • Olfactory Receptor Neurons / metabolism*
  • Pheromones / metabolism
  • Phylogeny
  • Receptors, Biogenic Amine / classification
  • Receptors, Biogenic Amine / genetics
  • Receptors, Biogenic Amine / metabolism*
  • Sequence Alignment
  • Smell / physiology

Substances

  • Pheromones
  • Receptors, Biogenic Amine
  • norsynephrine receptor
  • tyramine receptor