High quality structure of cleaved PAI-1-stab

J Struct Biol. 2009 Feb;165(2):126-32. doi: 10.1016/j.jsb.2008.11.001. Epub 2008 Nov 27.

Abstract

Here we report the crystal structure of a stablilized plasminogen activator inhibitor-1 variant (PAI-1-N150H-K154T-Q301P-Q319L-M354I (PAI-1-stab)) that shows a cleavage within the reactive centre loop. The new structure is of superior quality compared to the previously determined structure of the cleaved PAI-1-A335P mutant. We present a detailed comparison of the two structures and also compare them with the structure of the active PAI-1-stab. The structural data give important insights into the working mechanism of PAI-1 and also explain the role of various stabilizing mutations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalytic Domain
  • Crystallography, X-Ray
  • Humans
  • Models, Molecular
  • Molecular Conformation
  • Molecular Structure
  • Mutation
  • Plasminogen Activator Inhibitor 1 / genetics*
  • Plasminogen Activator Inhibitor 1 / physiology*
  • Protein Binding
  • Protein Conformation
  • Protein Structure, Tertiary
  • Serpins / chemistry

Substances

  • Plasminogen Activator Inhibitor 1
  • Serpins