From MEFs to matrigel I: passaging hESCs in the presence of MEFs

J Vis Exp. 2008 Jun 4:(16):722. doi: 10.3791/722.

Abstract

This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Collagen*
  • Drug Combinations
  • Embryo, Mammalian
  • Fibroblasts / cytology*
  • Humans
  • Laminin*
  • Mice
  • Neoplastic Stem Cells / cytology*
  • Proteoglycans*

Substances

  • Drug Combinations
  • Laminin
  • Proteoglycans
  • matrigel
  • Collagen