[Effects of constant magnetic fields on proliferation and migration of endothelial progenitor cells under rapamycin intervention: experiment with rats]

Zhonghua Yi Xue Za Zhi. 2008 Oct 21;88(38):2719-21.
[Article in Chinese]

Abstract

Objective: To investigate the effects of constant magnetic field (CMF) on proliferation and migration of bone marrow-derived endothelial progenitor cells (EPCs) under rapamycin intervention.

Methods: EPCs were isolated from rat bone marrow by density gradient centrifugation and cultured on fibronectin-coated dishes. Six days later the attached cells were divided into 5 groups: control group, rapamycin (1 ng/ml) group, and 3 rapamycin + CMF groups (treated with CMF of the doses 0.1 mT, 0.5 mT, and 1.0 mT respectively). Samples were collected 24 hours after incubation. Cell proliferation was measured by MTT chromatometre. EPC migration was detected with modified Boyden chamber assay.

Results: The EPC proliferation ability of the rapamycin group, expressed by absorbance, was (0.252 +/- 0.006), significantly lower than that of the control group [(0.328 +/- 0.025), P < 0.05]. The number of migrating EPC was (31 +/- 3) cells, significantly lower than that of the control group [(48 +/- 5), P < 0.05]. The EPC proliferation ability of the rapamycin + CMF 0.5 mT and 1.0 mT groups, expressed by absorbance, were (0.278 +/- 0.008) and (0.280 +/- 0.010) respectively, both significantly higher than that of the control group (both P < 0.05). The migrating EPC number of the rapamycin + CMF 0.5 mT and 1.0 mT groups were (37 +/- 3) and (38 +/- 4) respectively, both significantly higher than that of the control group (both P < 0.05).

Conclusion: CMF of the doses of 0.5 mT and 1.0 mT antagonizes the effects of rapamycin on EPCs, increasing the proliferation and migration of EPCs.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells / cytology
  • Cell Adhesion / radiation effects
  • Cell Differentiation
  • Cell Movement / radiation effects
  • Cell Proliferation / radiation effects
  • Cells, Cultured
  • Electromagnetic Fields*
  • Endothelial Cells / cytology
  • Endothelial Cells / drug effects
  • Endothelial Cells / radiation effects*
  • Rats
  • Rats, Sprague-Dawley
  • Sirolimus / pharmacology
  • Stem Cells / cytology
  • Stem Cells / drug effects
  • Stem Cells / radiation effects*

Substances

  • Sirolimus