This study examines the genotypes and allele frequency distributions of 21 forensic autosomal STRs for 7 populations from Yunnan province, China: Han (two different population samples, n=48 and n=59, respectively), Nu (n=36), Tibetan (n=31), Du Long (n=24), Lisu (n=25) and Yi (n=24). Pairwise FST analysis shows (marginally) significant differences between some of the Yunnan populations, but not between the Yunnan populations and other population samples from China and surrounding countries.