Quantitative matrix-assisted laser desorption/ionization mass spectrometry

Mark W Duncan; Heinrich Roder; Stephen W Hunsucker

doi:10.1093/bfgp/eln041

Quantitative matrix-assisted laser desorption/ionization mass spectrometry

Brief Funct Genomic Proteomic. 2008 Sep;7(5):355-70. doi: 10.1093/bfgp/eln041.

Authors

Mark W Duncan¹, Heinrich Roder, Stephen W Hunsucker

Affiliation

¹ Division of Endocrinology, Metabolism and Diabetes, School of Medicine, University of Colorado Denver, Mail Stop 8106, 12801 East 17th Avenue, Aurora, CO 80045, USA. mark.duncan@ucdenver.edu

Abstract

This review summarizes the essential characteristics of matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (TOF MS), especially as they relate to its applications in quantitative analysis. Approaches to quantification by MALDI-TOF MS are presented and published applications are critically reviewed.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

MeSH terms

Reproducibility of Results
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*