Inhibition of the SUMO pathway by Gam1

Mariaelena Pozzebon; Chiara V Segré; Susanna Chiocca

doi:10.1007/978-1-59745-566-4_19

Inhibition of the SUMO pathway by Gam1

Methods Mol Biol. 2009:497:285-301. doi: 10.1007/978-1-59745-566-4_19.

Authors

Mariaelena Pozzebon¹, Chiara V Segré, Susanna Chiocca

Affiliation

¹ FIRC Institute of Molecular Oncology, European Institute of Oncology (IFOM-IEO), Milan, Italy.

PMID: 19107425
DOI: 10.1007/978-1-59745-566-4_19

Abstract

We have previously demonstrated that Gam1, an avian adenoviral protein inhibits sumoylation. By counteracting the SUMO pathway, Gam1 has a significant impact on virus-infected cells, but in isolation the inhibitory effects of the Gam1 protein can be exploited to intentionally manipulate the SUMO system in vivo or in vitro. Here we discuss in detail the techniques we use to inhibit the SUMO pathway using the Gam1 protein.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Cells, Cultured
Clinical Laboratory Techniques
Humans
Protein Processing, Post-Translational / drug effects
Recombinant Proteins / isolation & purification
Recombinant Proteins / pharmacology
SUMO-1 Protein / antagonists & inhibitors*
SUMO-1 Protein / metabolism
Signal Transduction / drug effects*
Viral Proteins / isolation & purification
Viral Proteins / pharmacology*

Substances

CELO protein, adenovirus
Recombinant Proteins
SUMO-1 Protein
Viral Proteins