Alzheimer disease macrophages shuttle amyloid-beta from neurons to vessels, contributing to amyloid angiopathy

Acta Neuropathol. 2009 Feb;117(2):111-24. doi: 10.1007/s00401-008-0481-0. Epub 2009 Jan 13.

Abstract

Neuronal accumulation of oligomeric amyloid-beta (Alphabeta) is considered the proximal cause of neuronal demise in Alzheimer disease (AD) patients. Blood-borne macrophages might reduce Abeta stress to neurons by immigration into the brain and phagocytosis of Alphabeta. We tested migration and export across a blood-brain barrier model, and phagocytosis and clearance of Alphabeta by AD and normal subjects' macrophages. Both AD and normal macrophages were inhibited in Alphabeta export across the blood-brain barrier due to adherence of Abeta-engorged macrophages to the endothelial layer. In comparison to normal subjects' macrophages, AD macrophages ingested and cleared less Alphabeta, and underwent apoptosis upon exposure to soluble, protofibrillar, or fibrillar Alphabeta. Confocal microscopy of stained AD brain sections revealed oligomeric Abeta in neurons and apoptotic macrophages, which surrounded and infiltrated congophilic microvessels, and fibrillar Abeta in plaques and microvessel walls. After incubation with AD brain sections, normal subjects' monocytes intruded into neurons and uploaded oligomeric Abeta. In conclusion, in patients with AD, macrophages appear to shuttle Abeta from neurons to vessels where their apoptosis may release fibrillar Abeta, contributing to cerebral amyloid angiopathy.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Alzheimer Disease / pathology
  • Alzheimer Disease / physiopathology*
  • Amyloid beta-Peptides / chemistry
  • Amyloid beta-Peptides / metabolism*
  • Apoptosis
  • Blood-Brain Barrier
  • Brain / blood supply
  • Brain / pathology
  • Cerebral Amyloid Angiopathy / pathology
  • Cerebral Amyloid Angiopathy / physiopathology
  • Humans
  • Macrophages / metabolism*
  • Macrophages / pathology
  • Microvessels / metabolism*
  • Microvessels / pathology
  • Middle Aged
  • Models, Biological
  • Monocytes / physiology
  • Neurons / metabolism*
  • Neurons / pathology
  • Phagocytosis
  • Plaque, Amyloid / metabolism
  • Plaque, Amyloid / pathology
  • Young Adult

Substances

  • Amyloid beta-Peptides