Abstract
We performed chromatin immunoprecipitation-microarray analysis to investigate differences in function between StpA and H-NS in Escherichia coli cells. StpA binding regions essentially overlap those of H-NS in wild-type cells, while they are reduced to one-third in the hns mutant. The H-NS binding profile was unaffected by stpA inactivation.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Binding Sites
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Chromatin Immunoprecipitation
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Molecular Chaperones / genetics
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Molecular Chaperones / metabolism*
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Mutation*
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Oligonucleotide Array Sequence Analysis
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Protein Binding
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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Escherichia coli Proteins
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H-NS protein, bacteria
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Molecular Chaperones
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StpA protein, E coli