Abstract
Over ten different mammalian genes related to the Drosophila Shaker gene (the Sh gene family) have been identified recently. These genes encode subunits of voltage-dependent K+ channels. The family consists of four subfamilies: ShI genes are homologues of Shaker; ShII, ShIII, and ShIV are homologues of three other Shaker-like genes in Drosophila, Shab, Shaw, and Shal, respectively. We report here the cloning of a human K+ channel ShIII cDNA (HKShIIIC) obtained from a brain stem cDNA library. HKShIIIC transcripts express an atypical voltage-dependent transient (A-type) K+ current in Xenopus oocytes. This current is activated by large membrane depolarizations and is extremely sensitive to the K+ channel blocker TEA unlike most A-type currents. The gene encoding HKShIIIC maps to chromosome 1p21.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain Stem / physiology*
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Cerebellum / physiology*
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Chromosome Banding
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Chromosome Mapping
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Chromosomes, Human, Pair 1*
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Cloning, Molecular
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DNA / genetics*
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Drosophila / genetics
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Humans
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Ion Channel Gating
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Karyotyping
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Membrane Potentials / drug effects
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Molecular Sequence Data
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Multigene Family
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Oocytes / drug effects
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Oocytes / physiology*
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Potassium Channels / drug effects
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Potassium Channels / genetics*
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Potassium Channels / physiology
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Protein Conformation
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Sequence Homology, Nucleic Acid
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Tetraethylammonium
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Tetraethylammonium Compounds / pharmacology*
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Transcription, Genetic
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Xenopus
Substances
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Potassium Channels
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Tetraethylammonium Compounds
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Tetraethylammonium
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DNA