Cloning of a human cDNA expressing a high voltage-activating, TEA-sensitive, type-A K+ channel which maps to chromosome 1 band p21

J Neurosci Res. 1991 Jul;29(3):401-12. doi: 10.1002/jnr.490290316.

Abstract

Over ten different mammalian genes related to the Drosophila Shaker gene (the Sh gene family) have been identified recently. These genes encode subunits of voltage-dependent K+ channels. The family consists of four subfamilies: ShI genes are homologues of Shaker; ShII, ShIII, and ShIV are homologues of three other Shaker-like genes in Drosophila, Shab, Shaw, and Shal, respectively. We report here the cloning of a human K+ channel ShIII cDNA (HKShIIIC) obtained from a brain stem cDNA library. HKShIIIC transcripts express an atypical voltage-dependent transient (A-type) K+ current in Xenopus oocytes. This current is activated by large membrane depolarizations and is extremely sensitive to the K+ channel blocker TEA unlike most A-type currents. The gene encoding HKShIIIC maps to chromosome 1p21.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain Stem / physiology*
  • Cerebellum / physiology*
  • Chromosome Banding
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1*
  • Cloning, Molecular
  • DNA / genetics*
  • Drosophila / genetics
  • Humans
  • Ion Channel Gating
  • Karyotyping
  • Membrane Potentials / drug effects
  • Molecular Sequence Data
  • Multigene Family
  • Oocytes / drug effects
  • Oocytes / physiology*
  • Potassium Channels / drug effects
  • Potassium Channels / genetics*
  • Potassium Channels / physiology
  • Protein Conformation
  • Sequence Homology, Nucleic Acid
  • Tetraethylammonium
  • Tetraethylammonium Compounds / pharmacology*
  • Transcription, Genetic
  • Xenopus

Substances

  • Potassium Channels
  • Tetraethylammonium Compounds
  • Tetraethylammonium
  • DNA

Associated data

  • GENBANK/M64676