Molecular recognition by contact angle: proof of concept with DNA hybridization

Langmuir. 2009 Apr 21;25(8):4271-3. doi: 10.1021/la900428u.

Abstract

A molecular recognition reaction supported by a solid-phase assay drives a specific change in the solid-solution interfacial tension. This prompts contact angle (CA) analysis, being a straightforward route to evaluate this property, to play the unedited role of label-free probe of the reaction. The concept is proven by the successful recognition of DNA hybridization and is further supported by the agreement between the results and the underpinning thermodynamics.

MeSH terms

  • Biophysical Phenomena
  • Biophysics
  • Biosensing Techniques
  • Buffers
  • DNA / chemistry*
  • DNA Probes / chemistry*
  • DNA, Single-Stranded / chemistry
  • Kinetics
  • Ligands
  • Nucleic Acid Hybridization
  • Surface Plasmon Resonance
  • Surface Properties
  • Thermodynamics
  • Time Factors

Substances

  • Buffers
  • DNA Probes
  • DNA, Single-Stranded
  • Ligands
  • DNA