Utility of 16S ribosomal DNA sequencing in the diagnosis of Staphylococcus lugdunensis native valve infective endocarditis: case report and literature review

Surinder Pada; David C Lye; Yee Sin Leo; Timothy Barkham

doi:10.1016/j.ijid.2009.02.019

Utility of 16S ribosomal DNA sequencing in the diagnosis of Staphylococcus lugdunensis native valve infective endocarditis: case report and literature review

Int J Infect Dis. 2009 Nov;13(6):e511-3. doi: 10.1016/j.ijid.2009.02.019. Epub 2009 May 17.

Authors

Surinder Pada¹, David C Lye, Yee Sin Leo, Timothy Barkham

Affiliation

¹ Department of Infectious Diseases, Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng, Singapore 308433. surinder_pada_kaur@ttsh.com.sg

PMID: 19447659
DOI: 10.1016/j.ijid.2009.02.019

Abstract

We report a case of possible infective endocarditis without fever presenting with an acutely ischemic limb with prior antimicrobial therapy preventing identification by culture of a microorganism. 16S ribosomal DNA sequencing led to the identification of Staphylococcus lugdunensis from an embolus removed at surgery and subsequent successful antibiotic treatment. We review the utility of 16S ribosomal DNA sequencing in diagnosing infective endocarditis and other infectious conditions.

Publication types

Case Reports
Review

MeSH terms

Adult
DNA, Bacterial / analysis
DNA, Ribosomal / analysis
Endocarditis, Bacterial / diagnosis*
Endocarditis, Bacterial / microbiology
Heart Valve Diseases / diagnosis
Heart Valve Diseases / microbiology
Heart Valves / microbiology*
Humans
Male
RNA, Ribosomal, 16S / genetics*
Sequence Analysis, DNA / methods*
Staphylococcal Infections / diagnosis*
Staphylococcal Infections / microbiology
Staphylococcus / classification
Staphylococcus / genetics
Staphylococcus / isolation & purification

Substances

DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S