Mycobacterium tuberculosis in Ontario, Canada: Insights from IS6110 restriction fragment length polymorphism and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping

J Clin Microbiol. 2009 Aug;47(8):2651-4. doi: 10.1128/JCM.01946-08. Epub 2009 Jun 3.

Abstract

A collection of 1,308 clinical Mycobacterium tuberculosis isolates from Ontario, Canada, was genotyped by IS6110 restriction fragment length polymorphism (RFLP) and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) analysis. RFLP or >12 MIRU-VNTR loci were necessary for resolution of Indo-Oceanic strains. The low clustering rate and high strain diversity indicate that, in Ontario, most tuberculosis results from reactivation of latent infections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques / methods*
  • Cluster Analysis
  • DNA Fingerprinting / methods*
  • DNA Transposable Elements
  • DNA, Bacterial / genetics*
  • Genotype
  • Humans
  • Minisatellite Repeats*
  • Molecular Epidemiology
  • Mycobacterium tuberculosis / classification*
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / isolation & purification*
  • Ontario / epidemiology
  • Polymorphism, Genetic
  • Polymorphism, Restriction Fragment Length*
  • Tuberculosis / epidemiology*

Substances

  • DNA Transposable Elements
  • DNA, Bacterial