Antiviral resistance in influenza viruses circulating in Central and South America based on the detection of established genetic markers

Influenza Other Respir Viruses. 2009 Mar;3(2):69-74. doi: 10.1111/j.1750-2659.2009.00072.x.

Abstract

Background: Recent influenza antiviral resistance studies reveal an alarming increase in both adamantanes and neuraminidase inhibitors (NAIs) resistant viral strains worldwide, particularly in Asia, Europe and the United States.

Objectives: In this study, we have evaluated influenza virus resistance in Central and South America.

Methods: Influenza viruses, isolated from symptomatic patients throughout Central and South America in 2005-2008 were analyzed for inhibitor resistance. The M2 and NA genes of influenza viruses were sequenced and resistance was inferred by comparison with published sequences and known resistant mutations.

Results: Our results indicate that: (i) resistance to adamantanes was seen in the majority (95.5%) of the influenza A/H3N2 isolates but only in one isolate of the influenza A/H1N1 viruses; (ii) resistance to NAIs began to be detected in A/H1N1 isolates from Central America in 2008; and (iii) none of the influenza B viruses analyzed were resistant to NAIs.

Conclusions: These findings suggest a limited effectiveness of influenza inhibitors due to the detection of resistance among A/H1 and A/H3 viruses.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adamantane / pharmacology
  • Adamantane / therapeutic use
  • Amino Acid Sequence
  • Animals
  • Antiviral Agents / pharmacology*
  • Antiviral Agents / therapeutic use
  • Cell Line
  • Central America
  • Dogs
  • Drug Resistance, Viral*
  • Female
  • Humans
  • Influenza A Virus, H1N1 Subtype / classification
  • Influenza A Virus, H1N1 Subtype / drug effects
  • Influenza A Virus, H1N1 Subtype / genetics
  • Influenza A Virus, H1N1 Subtype / isolation & purification
  • Influenza A Virus, H3N2 Subtype / classification
  • Influenza A Virus, H3N2 Subtype / drug effects
  • Influenza A Virus, H3N2 Subtype / genetics
  • Influenza A Virus, H3N2 Subtype / isolation & purification
  • Influenza, Human / drug therapy
  • Influenza, Human / virology*
  • Male
  • Molecular Sequence Data
  • Neuraminidase / antagonists & inhibitors
  • Neuraminidase / chemistry
  • Neuraminidase / genetics*
  • Orthomyxoviridae / classification
  • Orthomyxoviridae / drug effects*
  • Orthomyxoviridae / genetics
  • Orthomyxoviridae / isolation & purification
  • Oseltamivir / pharmacology
  • Oseltamivir / therapeutic use
  • Sequence Alignment
  • South America
  • Viral Matrix Proteins / chemistry
  • Viral Matrix Proteins / genetics*

Substances

  • Antiviral Agents
  • Viral Matrix Proteins
  • Oseltamivir
  • Neuraminidase
  • Adamantane