Objective: To observe the effects of volatile oil of Rhizoma Acori Tatarinowii (VOSCP) on morphology and cell viability in cultured neonate rat cardiac myocytes.
Methods: The cardiac myocytes were cultured by trypsin under cool treatment, and the cell purity was assayed with imminocytochemistry; Morphological changes were observed under phase contrast microscope after cardiac myocytes with VOSCP of different concentration for 24h in vitro, and the cell viability was examed by MTT assay.
Results: The purity of cultured neonate rat cardiac myocytes was higher than 95%; VOSCP of different concentration could depress pulse frequency and 100-160 mg/L VOSCP can obviously improve the viability of cardiac myocytes. The relation of dose-effect relationship was a parabola whose peak was at the value of 140 mg/L.
Conclusion: VOSCP of proper concentration can depress pulse frequency and improve the viability of cardiac myocytes.