Abstract
A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Camphor 5-Monooxygenase / chemistry*
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Camphor 5-Monooxygenase / genetics*
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Oxidoreductases / chemistry*
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Oxidoreductases / genetics*
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Protein Engineering / methods*
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Protein Structure, Tertiary
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Recombinant Fusion Proteins / chemistry*
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Recombinant Fusion Proteins / genetics*
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Rhodococcus / enzymology
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Transformation, Genetic
Substances
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Recombinant Fusion Proteins
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Oxidoreductases
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Camphor 5-Monooxygenase