Detection and quantification of hepatitis C virus (HCV) by MultiCode-RTx real-time PCR targeting the HCV 3' untranslated region

Elizabeth K Mulligan; J J Germer; Max Q Arens; Krista L D'Amore; Adrian Di Bisceglie; Nathan A Ledeboer; Michael J Moser; Andrew C Newman; Andy K O'Guin; Paul D Olivo; Diane S Podzorski; Kimberly A Vaughan; Joseph D Yao; Slava A Elagin; Scott C Johnson

doi:10.1128/JCM.02170-08

Detection and quantification of hepatitis C virus (HCV) by MultiCode-RTx real-time PCR targeting the HCV 3' untranslated region

J Clin Microbiol. 2009 Aug;47(8):2635-8. doi: 10.1128/JCM.02170-08. Epub 2009 Jun 17.

Authors

Elizabeth K Mulligan¹, J J Germer, Max Q Arens, Krista L D'Amore, Adrian Di Bisceglie, Nathan A Ledeboer, Michael J Moser, Andrew C Newman, Andy K O'Guin, Paul D Olivo, Diane S Podzorski, Kimberly A Vaughan, Joseph D Yao, Slava A Elagin, Scott C Johnson

Affiliation

¹ EraGen Biosciences, Inc., Madison, WI 53717, USA.

Abstract

A prototype, real-time reverse-transcription PCR assay, based on MultiCode-RTx technology, quantifying hepatitis C virus (HCV) RNA by targeting the HCV 3' untranslated region demonstrated linearity over 7 logs, with a good correlation between the quantitative results of this assay and the results of two commercially available comparator assays for 466 clinical specimens comprising all six HCV genotypes.

Publication types

Comparative Study
Evaluation Study

MeSH terms

3' Untranslated Regions*
Hepacivirus / genetics
Hepacivirus / isolation & purification*
Hepatitis C / diagnosis*
Hepatitis C / virology
Humans
Plasma / virology
RNA, Viral / genetics*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Serum / virology
Viral Load / methods*

Substances

3' Untranslated Regions
RNA, Viral