[MyoD mRNA expression in skeletal muscle of patients with myotonic dystrophy]

Zhonghua Yi Xue Za Zhi. 2009 Feb 24;89(7):466-8.
[Article in Chinese]

Abstract

Objective: To explore the mRNA expression of MyoD gene in the skeletal muscles of myotonic dystrophy (MD) patients.

Methods: Muscle biopsy specimens were obtained from the biceps muscles of arm of 4 MD patients and 4 healthy controls. Semi-quantitative reverse transcription polymerase chain reaction was performed to evaluate the mRNA expression of MyoD in the specimens.

Results: The mRNA expression index of MyoD of the MD patients was (0.267 +/- 0.114), significantly lower than that of the healthy controls [(0.788 +/- 0.136), P = 0.001].

Conclusion: Down-regulated mRNA expression of MyoD gene is involved in the mechanism of DM.

Publication types

  • English Abstract

MeSH terms

  • Adolescent
  • Adult
  • Case-Control Studies
  • Child
  • Female
  • Gene Expression
  • Humans
  • Male
  • Middle Aged
  • Muscle, Skeletal / metabolism*
  • MyoD Protein / genetics*
  • Myotonic Dystrophy / genetics*
  • Myotonic Dystrophy / metabolism
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Young Adult

Substances

  • MyoD Protein
  • MyoD1 myogenic differentiation protein
  • RNA, Messenger