Modified low density lipoproteins activate human macrophages to secrete immunoreactive endothelin

FEBS Lett. 1991 Nov 18;293(1-2):127-30. doi: 10.1016/0014-5793(91)81167-7.

Abstract

This study attempted to determine if low density lipoproteins (LDL) induce the production of endothelins (ET) by human macrophages. Non-protected LDL from macrophage induced oxidation (n-LDL), copper-oxidized LDL (Ox-LDL), acetylated-LDL (Ac-LDL), butylated hydroxytoluene-LDL (BHT-LDL), BHT-Ac-LDL, polyinosinic acid (PiA, 1.5 micrograms/ml), phorbol myristate acetate (PMA; 0.5 microM) and BHT alone (20 microM) were studied. The different compounds had the following potency to stimulate the ET secretion: PMA greater than Ox-LDL greater than Ac-LDL greater than n-LDL greater than BHT-LDL greater than PiA greater than PiA + Ac-LDL greater than BHT. In conclusion, modified LDL stimulated ET secretion by human macrophages.

MeSH terms

  • Binding Sites
  • Cells, Cultured
  • Endothelins / immunology
  • Endothelins / metabolism*
  • Humans
  • Lipoproteins, LDL / pharmacology*
  • Macrophage Activation / drug effects*
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Tetradecanoylphorbol Acetate / pharmacology

Substances

  • Endothelins
  • Lipoproteins, LDL
  • Tetradecanoylphorbol Acetate