Connecting viral with cellular interactomes

Curr Opin Microbiol. 2009 Aug;12(4):453-9. doi: 10.1016/j.mib.2009.06.004. Epub 2009 Jul 24.

Abstract

Genome-scale screens for intraviral and virus-host protein interactions and the analysis of literature-curated datasets are able to provide a novel, comprehensive perspective of viruses, and virus-infected cells. Until now, large-scale interaction screens were predominantly performed with the yeast-two-hybrid (Y2H) system; however, alternative high-throughput technologies detecting binary protein interactions or protein complexes have been developed. Although many of the previous studies suffer from a rather poor validation of the results and few biological implications, these technologies potentially lead to a plethora of novel hypotheses. Here, we will give an overview of current approaches and their technical limitations, present recent examples and novel developments.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cells / immunology
  • Cells / virology
  • Gene Expression Profiling / methods
  • Genomics / methods
  • Host-Pathogen Interactions*
  • Protein Interaction Mapping
  • Proteomics / methods
  • Systems Biology / methods*
  • Two-Hybrid System Techniques
  • Viruses / growth & development
  • Viruses / immunology