Introduction of an aliphatic ketone into recombinant proteins in a bacterial strain that overexpresses an editing-impaired leucyl-tRNA synthetase

Chembiochem. 2009 Sep 4;10(13):2188-90. doi: 10.1002/cbic.200900407.

Abstract

A leucine analog containing a ketone has been incorporated into proteins in E. coli. Only E. coli strains overexpressing an editing-deficient leucyl-tRNA synthetase were capable of synthesizing proteins with the aliphatic ketone amino acid. Modification of ketone-containing proteins under mild conditions has been demonstrated.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Escherichia coli / enzymology*
  • Ketones / chemistry*
  • Ketones / metabolism
  • Leucine / chemistry
  • Leucine / metabolism
  • Leucine-tRNA Ligase / genetics
  • Leucine-tRNA Ligase / metabolism*
  • Protein Modification, Translational
  • RNA, Transfer, Amino Acyl / metabolism
  • Recombinant Proteins / chemistry*
  • Recombinant Proteins / metabolism

Substances

  • Ketones
  • RNA, Transfer, Amino Acyl
  • Recombinant Proteins
  • Leucine-tRNA Ligase
  • Leucine