Isolation of deletion alleles by G4 DNA-induced mutagenesis

Daphne B Pontier; Evelien Kruisselbrink; Victor Guryev; Marcel Tijsterman

doi:10.1038/nmeth.1362

Isolation of deletion alleles by G4 DNA-induced mutagenesis

Nat Methods. 2009 Sep;6(9):655-7. doi: 10.1038/nmeth.1362. Epub 2009 Aug 16.

Authors

Daphne B Pontier¹, Evelien Kruisselbrink, Victor Guryev, Marcel Tijsterman

Affiliation

¹ Hubrecht Institute-Koninklijke Nederlandse Akademie van Wetenschappen, University Medical Centre Utrecht, Utrecht, The Netherlands.

PMID: 19684597
DOI: 10.1038/nmeth.1362

Abstract

Metazoan genomes contain thousands of sequence tracts that match the guanine-quadruplex (G4) DNA signature G(3)N(x)G(3)N(x)G(3)N(x)G(3), a motif that is intrinsically mutagenic, probably because it can form secondary structures during DNA replication. Here we show how and to what extent this feature can be used to generate deletion alleles of many Caenorhabditis elegans genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Animals
Caenorhabditis elegans / genetics*
DNA / chemistry
DNA / genetics*
G-Quadruplexes*
Gene Deletion*
Mutagenesis*

Substances

DNA