Two important components of mating behavior, mating latency and duration of copulation, were examined during first and second mating in D. melanogaster females. Our results confirm the published findings of lower receptivity of previously mated D. melanogaster females, since fertilized females present significantly longer mating latencies. Duration of copulation also depends on female mating experience, as naive females copulated significantly longer than those previously mated. An important role of female D. melanogaster in the control of both examined components of mating behavior is displayed in the course of second mating, as part of behavioral and physiological changes induced by the first mating.