Regulation of guanylate cyclase activity by atrial natriuretic factor and protein kinase C

Mol Cell Biochem. 1990 Mar 27;93(2):179-84. doi: 10.1007/BF00226190.

Abstract

The putative 'second messenger' of certain atrial natriuretic factor (ANF) signal transductions is cyclic GMP. Recently, we purified a 180-kDa protein, apparently containing both ANF receptor and guanylate cyclase activities, and hypothesized that this is one of the cyclic GMP transmembrane signal transducers. The enzyme is ubiquitous and appears to be conserved. Utilizing the 180-kDa membrane guanylate cyclase, we now show that the 180-kDa guanylate cyclase is regulated in opposing fashions by two receptor signals--ANF stimulating it and protein kinase C inhibiting it. Furthermore, protein kinase C phosphorylates the 180-kDa enzyme. This suggests a novel 'switch on' and 'switch off' mechanism of the cyclic GMP signal transduction. 'Switch off' represents the phosphorylation while 'switch on' the dephosphorylation of the enzyme.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Atrial Natriuretic Factor / pharmacology*
  • Cell Membrane / drug effects
  • Cell Membrane / enzymology
  • Cyclic GMP / metabolism
  • Guanylate Cyclase / metabolism*
  • Molecular Weight
  • Phosphorylation
  • Protein Kinase C / pharmacology*
  • Rats
  • Signal Transduction
  • Tumor Cells, Cultured

Substances

  • Atrial Natriuretic Factor
  • Protein Kinase C
  • Guanylate Cyclase
  • Cyclic GMP