Multi-locus variable number tandem repeat analysis for Escherichia coli causing extraintestinal infections

J Microbiol Methods. 2009 Nov;79(2):211-3. doi: 10.1016/j.mimet.2009.09.006. Epub 2009 Sep 16.

Abstract

Discriminatory genotyping methods for the analysis of Escherichia coli other than O157:H7 are necessary for public health-related activities. A new multi-locus variable number tandem repeat analysis protocol is presented; this method achieves an index of discrimination of 99.5% and is reproducible and valid when tested on a collection of 836 diverse E. coli.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Typing Techniques / methods*
  • Cluster Analysis
  • DNA Fingerprinting / methods*
  • DNA, Bacterial / genetics*
  • Escherichia coli / classification*
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / microbiology*
  • Genotype
  • Humans
  • Meat / microbiology*
  • Minisatellite Repeats*
  • Molecular Epidemiology / methods
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • DNA, Bacterial