Effect of calcium-modulating cyclophilin ligand on human immunodeficiency virus type 1 particle release and cell surface expression of tetherin

J Virol. 2009 Dec;83(24):13032-6. doi: 10.1128/JVI.01786-09. Epub 2009 Sep 30.

Abstract

The human immunodeficiency virus type 1 (HIV-1) accessory protein Vpu enhances virus particle release by counteracting a host factor that retains virions at the surfaces of infected cells. It was recently demonstrated that cellular protein BST-2/CD317/Tetherin restricts HIV-1 release in a Vpu-dependent manner. Calcium-modulating cyclophilin ligand (CAML) was also proposed to be involved in this process. We investigated whether CAML is involved in cell surface expression of Tetherin. Here, we show that CAML overexpression in permissive Cos-7 cells or CAML depletion in restrictive HeLa cells has no effect on HIV-1 release or on Tetherin surface expression, indicating that CAML is not required for Tetherin-mediated restriction of HIV-1 release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / antagonists & inhibitors
  • Adaptor Proteins, Signal Transducing / physiology*
  • Animals
  • Antigens, CD / analysis
  • Antigens, CD / physiology*
  • COS Cells
  • Chlorocebus aethiops
  • GPI-Linked Proteins
  • HIV-1 / physiology*
  • HeLa Cells
  • Humans
  • Membrane Glycoproteins / analysis
  • Membrane Glycoproteins / physiology*
  • Virion / physiology*

Substances

  • Adaptor Proteins, Signal Transducing
  • Antigens, CD
  • BST2 protein, human
  • CAMLG protein, human
  • GPI-Linked Proteins
  • Membrane Glycoproteins