Abstract
We developed a general method to detect cellular small molecule-RNA conjugates that does not rely on the reactivity of the small molecule. This technique revealed NAD-linked RNA in Escherichia coli and Streptomyces venezuelae. Subsequent characterization showed that NAD is a 5' modification of RNA, cannot be installed in vitro through aberrant transcriptional initiation, is only found among smaller cellular RNAs and is present at a surprisingly high abundance of approximately 3,000 copies per cell.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Chromatography, High Pressure Liquid
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Escherichia coli / chemistry*
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Escherichia coli / enzymology
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Escherichia coli / metabolism
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Mass Spectrometry
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NAD / isolation & purification*
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NAD / metabolism
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RNA, Bacterial / isolation & purification*
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RNA, Bacterial / metabolism
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RNA, Fungal / isolation & purification*
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RNA, Fungal / metabolism
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RNA, Transfer / isolation & purification*
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RNA, Transfer / metabolism
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Streptomyces / chemistry*
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Streptomyces / enzymology
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Streptomyces / metabolism
Substances
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RNA, Bacterial
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RNA, Fungal
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NAD
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RNA, Transfer
Associated data
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PubChem-Substance/85195596
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PubChem-Substance/85195597
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PubChem-Substance/85195598
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PubChem-Substance/85195599