The potential for circular dichroism as an additional facile and sensitive method of monitoring low-molecular-weight heparins and heparinoids

Thromb Haemost. 2009 Nov;102(5):874-8. doi: 10.1160/TH08-12-0797.

Abstract

The ultraviolet circular dichroism (CD) spectra of commercial low-molecular-weight heparins, heparinoids and other anticoagulant preparations have been recorded between 180 and 260 nm. Principal component analysis of the spectra allowed their differentiation into a number of groups related to the means of their production reflecting the structural changes introduced by each process. The findings suggest that CD provides a complementary technique for the rapid analysis of heparin preparations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anticoagulants / chemistry*
  • Anticoagulants / isolation & purification
  • Cattle
  • Circular Dichroism*
  • Drug Monitoring / methods*
  • Factor Analysis, Statistical
  • Glycosaminoglycans / chemistry
  • Heparin, Low-Molecular-Weight / chemistry*
  • Heparin, Low-Molecular-Weight / isolation & purification
  • Heparinoids / chemistry*
  • Heparinoids / classification
  • Heparinoids / isolation & purification
  • Molecular Structure
  • Multivariate Analysis
  • Principal Component Analysis
  • Swine

Substances

  • Anticoagulants
  • Glycosaminoglycans
  • Heparin, Low-Molecular-Weight
  • Heparinoids