Use of a baculovirus-expressed structural protein for the detection of antibodies to foot-and-mouth disease virus type A by a blocking enzyme-linked immunosorbent assay

Clin Vaccine Immunol. 2010 Jan;17(1):194-8. doi: 10.1128/CVI.00374-09. Epub 2009 Nov 4.

Abstract

A blocking enzyme-linked immunosorbent assay (ELISA) with a baculovirus-expressed structural protein was developed for the detection of antibodies to foot-and-mouth disease virus type A. It exhibited 99% specificity with a cutoff of 53% inhibition. Its sensitivity was comparable to the sensitivities of the virus neutralization test and the liquid-phase blocking ELISA, indicating its potential as an alternative assay.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Antigens, Viral* / genetics
  • Baculoviridae / genetics*
  • Cattle
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Foot-and-Mouth Disease Virus / immunology*
  • Genetic Vectors*
  • Goats
  • Recombinant Proteins / genetics
  • Sensitivity and Specificity
  • Swine

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Recombinant Proteins