A DNA-based electrochemical strategy for label-free monitoring the activity and inhibition of protein kinase

Chem Commun (Camb). 2009 Dec 7:(45):6946-8. doi: 10.1039/b913943e. Epub 2009 Oct 16.

Abstract

A novel label-free electrochemical strategy for monitoring the activity and inhibition of protein kinase is developed, based on the linkage between the phosphorylated peptide and DNA functionalized Au nanoparticles (DNA-AuNPs) by Zr(4+) and the chronocoulometric response of [Ru(NH(3))(6)](3+) absorbed on the DNA-AuNPs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • DNA / chemistry*
  • Electrochemical Techniques
  • Gold / chemistry
  • Immobilized Proteins / chemistry
  • Immobilized Proteins / metabolism
  • Metal Nanoparticles / chemistry
  • Phosphorylation
  • Protein Kinases / chemistry
  • Protein Kinases / metabolism*
  • Ruthenium / chemistry
  • Zirconium / chemistry

Substances

  • Immobilized Proteins
  • Gold
  • Ruthenium
  • DNA
  • Zirconium
  • Protein Kinases