zeta-Crystallin is a major protein in the lens of Camelus dromedarius

Arch Biochem Biophys. 1991 Feb 15;285(1):134-6. doi: 10.1016/0003-9861(91)90339-k.

Abstract

Camel (Camelus dromedarius) lenses contain a protein with an apparent subunit Mr 38,000 that constitutes approximately 8-13% of the total protein. The protein has been purified and has a native Mr 140,000 as determined by gel filtration. This is consistent with its being a tetramer. The protein reacts with antibodies raised against both guinea pig zeta-crystallin and peptides corresponding to amino acids 1-10 and 295-308, but not to antibodies raised against amino acids 320-328 of zeta-crystallin. Based on these criteria it is concluded that this protein, which is a major constituent of camel lens, is zeta-crystallin. This may be the first example of a protein (enzyme) being independently utilized as a crystallin in the lens of species from two mammalian orders.

MeSH terms

  • Animals
  • Antibodies / immunology
  • Camelus
  • Chromatography, Gel
  • Crystallins / analysis*
  • Crystallins / immunology
  • Lens, Crystalline / chemistry*
  • Molecular Weight

Substances

  • Antibodies
  • Crystallins