Probing the relevance of lectin clustering for the reliable evaluation of multivalent carbohydrate recognition

Eva Maria Munoz; Juan Correa; Eduardo Fernandez-Megia; Ricardo Riguera

doi:10.1021/ja9074826

Probing the relevance of lectin clustering for the reliable evaluation of multivalent carbohydrate recognition

J Am Chem Soc. 2009 Dec 16;131(49):17765-7. doi: 10.1021/ja9074826.

Authors

Eva Maria Munoz¹, Juan Correa, Eduardo Fernandez-Megia, Ricardo Riguera

Affiliation

¹ Departamento de Química Orgánica, Facultad de Química, and Unidad de RMN de Biomoléculas Asociada al CSIC, Universidad de Santiago de Compostela, Avda. de las Ciencias S.N. 15782 Santiago de Compostela, Spain.

PMID: 19919053
DOI: 10.1021/ja9074826

Abstract

Experiments by Surface Plasmon Resonance (SPR) illustrate the relevance of lectin density for the reliable evaluation of binding efficiencies in surface-based multivalent carbohydrate recognition. The difference between affinity data obtained by solution and surface-based experiments is also stressed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Concanavalin A / chemistry*
Dendrimers / chemistry
Gallic Acid / chemistry
Mannose / chemistry*
Molecular Structure
Polyethylene Glycols / chemistry
Surface Properties

Substances

Dendrimers
Concanavalin A
triethylene glycol
Polyethylene Glycols
Gallic Acid
Mannose