Long-term activation of the pro-coagulant response after neoadjuvant chemoradiation and major cancer surgery

Br J Cancer. 2010 Jan 5;102(1):73-9. doi: 10.1038/sj.bjc.6605463. Epub 2009 Dec 1.

Abstract

Background: The association between cancer, major surgery and venous thromboembolism (VTE) is well established. Multimodal therapy is increasingly being used as standard treatment for localised gastrointestinal cancer. The aim of this study was to examine the markers of pro-coagulation response and VTE risk in an exemplar multimodal model of pre-operative combination chemotherapy and radiation therapy, followed by complex cancer surgery.

Methods: Consecutive patients (n=36) with localised oesophageal cancer were studied at baseline after the first and second cycles of chemoradiation, and on post-operative days 1-28, and at 3, 6 and 9 months. Factors regulating the pro- and anti-coagulant response, as well as pro-inflammatory markers including NFkappaB activation in peripheral blood mononuclear cells, were examined. All patients received enoxaparin 40 mg s.c. postoperatively up to discharge, and underwent pulmonary CT-pulmonary angiography and venography on day 10 postoperatively.

Results: Four (11%) non-fatal thromboembolic events were documented, all after hospital discharge. Neoadjuvant therapy before surgery activated factor VIII (FVIII) and pro-inflammatory NFkappaB, and increased D-dimers, pro-thrombin fragment 1+2 (F1+2) and the thrombin-anti-thrombin complex (TAT). Surgery significantly (P<0.05) increased pro-thrombin time (PT), activated partial thromboplastin time, fibrinogen, D-dimers, TAT, F1+2 and FVIII up to 6 months.

Conclusion: These data highlight the linked pro-coagulant and immunoinflammatory pathways in the multimodal management of oesophageal cancer, and suggest that the duration of current standard thromboprophylaxis regimens warrants further study.

MeSH terms

  • Adenocarcinoma / blood*
  • Adenocarcinoma / drug therapy
  • Adenocarcinoma / immunology
  • Adenocarcinoma / radiotherapy
  • Adenocarcinoma / surgery
  • Adult
  • Aged
  • Anticoagulants / therapeutic use
  • Biomarkers
  • Carcinoma, Squamous Cell / blood*
  • Carcinoma, Squamous Cell / drug therapy
  • Carcinoma, Squamous Cell / immunology
  • Carcinoma, Squamous Cell / radiotherapy
  • Carcinoma, Squamous Cell / surgery
  • Combined Modality Therapy
  • Cytokines / blood
  • Enoxaparin / therapeutic use
  • Esophageal Neoplasms / blood*
  • Esophageal Neoplasms / drug therapy
  • Esophageal Neoplasms / immunology
  • Esophageal Neoplasms / radiotherapy
  • Esophageal Neoplasms / surgery
  • Esophagectomy*
  • Factor VIII / metabolism
  • Female
  • Fibrin Fibrinogen Degradation Products / analysis
  • Follow-Up Studies
  • Humans
  • Intercellular Signaling Peptides and Proteins / blood
  • Male
  • Middle Aged
  • NF-kappa B / metabolism
  • Neoadjuvant Therapy*
  • Neoplasm Proteins / metabolism
  • Postoperative Complications / blood
  • Postoperative Complications / chemically induced
  • Postoperative Complications / etiology*
  • Pulmonary Embolism / diagnostic imaging
  • Pulmonary Embolism / etiology
  • Thrombophilia / blood
  • Thrombophilia / chemically induced
  • Thrombophilia / etiology*
  • Tomography, X-Ray Computed
  • Venous Thromboembolism / etiology*

Substances

  • Anticoagulants
  • Biomarkers
  • Cytokines
  • Enoxaparin
  • Fibrin Fibrinogen Degradation Products
  • Intercellular Signaling Peptides and Proteins
  • NF-kappa B
  • Neoplasm Proteins
  • Factor VIII