Quantitative spot-test analysis of metformin in pharmaceutical preparations using ultraviolet-visible diffuse reflectance spectroscopy

Anal Sci. 2010 Jan;26(1):121-4. doi: 10.2116/analsci.26.121.

Abstract

A quantitative spot-test for the determination of metformin in pharmaceutical preparations using diffuse UV-visible reflectance is reported. The procedure is quite simple, involving in the formation of a metformin-nickel(II) complex on a glass filter membrane with a later measurement of the reflectance in the spectrophotometer using an integration sphere. The analytical results obtained with commercial products were statistically compared with those resulting from a method recommended by JP and by USP, where complete agreement was observed. The average RSD is 2.5% and the detection (0.009 mol L(-1)) and the quantitation (0.03 mol L(-1)) limits are quite adequate for pharmaceutical analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Filtration
  • Hypoglycemic Agents / analysis*
  • Indicators and Reagents
  • Membranes, Artificial
  • Metformin / analysis*
  • Nickel / chemistry
  • Potentiometry
  • Solutions
  • Spectrophotometry, Ultraviolet
  • Tablets / analysis

Substances

  • Hypoglycemic Agents
  • Indicators and Reagents
  • Membranes, Artificial
  • Solutions
  • Tablets
  • Nickel
  • Metformin