Transcriptional target-based expression cloning of immunoregulatory molecules

Immunol Res. 2010 Jul;47(1-3):172-8. doi: 10.1007/s12026-009-8148-z.

Abstract

Immunologic research has benefited tremendously from expression-cloning strategies designed to isolate genes responsible for a wide variety of immunomodulatory activities, including cytokines, receptors, signaling proteins, and transcription factors. Here, we discuss the use of expression-cloning strategies that have been modified to detect cDNAs that influence gene expression as assayed by a transcriptional reporter. We summarize our experience with these screens, review important parameters, and discuss potential modifications.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cell Line
  • Cloning, Molecular / methods*
  • Cytokines / genetics*
  • Cytokines / metabolism
  • Gene Expression Regulation*
  • Gene Library*
  • Genes, Reporter
  • HIV-1 / physiology
  • Humans
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcription, Genetic*

Substances

  • Cytokines
  • Transcription Factors