Introduction of pharmacogenetic screening for the human leucocyte antigen (HLA) B*5701 variant in Polish HIV-infected patients

HIV Med. 2010 May;11(5):345-8. doi: 10.1111/j.1468-1293.2009.00787.x. Epub 2010 Jan 11.

Abstract

Objective: Prospective pharmacogenetic screening for the human leucocyte antigen (HLA) B*5701 allele can significantly reduce the number of cases of abacavir-related hypersensitivity among HIV-infected patients treated with this drug. The aim of this study was to establish the frequency of the HLA B*5701 variant in HIV-infected Poles.

Methods: The sequence-specific primer (SSP) test was used to assess the feasibility of the introduction of such testing in clinical practice. For this purpose, 234 randomly selected HIV-positive patients were screened using a low-resolution SSP assay, with HLA B*5701-positive results confirmed using a high-resolution test.

Results and conclusions: The HLA B*5701 variant was found in 11 of 234 subjects (4.7%). Testing with the selected method proved quick and reliable.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Alleles
  • Dideoxynucleosides / adverse effects*
  • Drug Hypersensitivity / genetics*
  • Feasibility Studies
  • Female
  • Gene Frequency
  • Genetic Testing*
  • Genotype
  • HIV Infections / drug therapy*
  • HIV Infections / genetics
  • HLA-B Antigens / analysis
  • HLA-B Antigens / genetics*
  • Humans
  • Male
  • Middle Aged
  • Mutation
  • Poland
  • Polymerase Chain Reaction / methods
  • Predictive Value of Tests
  • Reverse Transcriptase Inhibitors / adverse effects*
  • Skin Tests

Substances

  • Dideoxynucleosides
  • HLA-B Antigens
  • HLA-B*57:01 antigen
  • Reverse Transcriptase Inhibitors
  • abacavir